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Old 05-18-2017, 12:39 PM   #5
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Location: Iran

Join Date: Jan 2013
Posts: 1,080

I think polyA is the result of library prep method. Some methods add a strech of A to ssDNA which is used to prime second strand synthesis or ligating adapters. If they have used a kit for library prep the supplier should have some recommendation regarding best sequencing approach.
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