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Old 09-09-2011, 09:09 AM   #4
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
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I guess picogreen should not work well. It is a double stranded fluor that fluoresces very weakly in the presence of single stranded molecules. Hence it is not confounded much by RNA contamination of a DNA sample. For short oligonucleotides anneal over a short stretch I don't know you would get much signal.

But if your concentration results were fairly linear over a set of dilutions, I guess you will be okay.

Anyway, no, we have not tried it ourselves. Please let us know how it turns out.

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Phillip
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