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Old 09-10-2011, 02:38 AM   #5
ScottC
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Location: Monash University, Melbourne, Australia.

Join Date: Jan 2008
Posts: 246
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Hi,

Yes, I realise PG detects primarily dsDNA, but I think the adapters have sufficiently large double stranded regions to work with picogreen. We really only need a relative concentration rather than an abolute concentration.

It appears that they're approximately 60 fold more dilute in the RNA-seq kit than in the genomic DNA kit.

We diluted the DNA adapters 1 in 50 and the preps appear to have worked well.

Cheers,

Scott.
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