SEQanswers - View Single Post - Custom barcoded RNA-seq

NextGenSeq · 07-30-2010, 09:28 AM

Note it is a very common problem to get uneven distribution of coverage in bar-coded libraries even using the Illumina adaptors. Thus if you are planning on expression analysis I doubt it will be valid.

07-30-2010, 09:28 AM	#2
NextGenSeq Senior Member Location: USA Join Date: Apr 2009 Posts: 482	Note it is a very common problem to get uneven distribution of coverage in bar-coded libraries even using the Illumina adaptors. Thus if you are planning on expression analysis I doubt it will be valid.