Quote:
Originally Posted by NextGenSeq
Note it is a very common problem to get uneven distribution of coverage in bar-coded libraries even using the Illumina adaptors. Thus if you are planning on expression analysis I doubt it will be valid.
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Not necessarily- expression analysis packages like edgeR and DESeq have library size correction factors built in to the statistics. If one library is severely under-represented though it will reduce the statistical power of your analysis.