View Single Post
Old 08-04-2010, 08:46 PM   #5
link1
Member
 
Location: US

Join Date: May 2010
Posts: 21
Default barcoding small RNAs

we tried barcoding the 5'RNA adapter and found significant bias in our small RNA preps. it turns out that RNA Lig I prefers some bases over others. You will need to barcode the 3' adenylated adapter because it utilizes a truncated version of RNA Ligase 2, which does not exhibit as much base preference.
You can design and make these yourself or purchase a set that has already been designed and optimized to eliminate bias. Initially I tried making these myself. Now I purchase the set below. I've done several runs now with multiplexed samples with good results.

http://www.biooscientific.com/Detail...tGenSequencing
link1 is offline   Reply With Quote