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Hedi86 · 01-31-2020, 04:57 AM

thank you Felix

so i just imported the BAM files generated by Bismark into Seqmonk, without any quantification i can click on read length distribution histogram on data sets. but trimmed files should have 20 - 140 bp length, why still i can see seq with for example 400 bp in BAM files?

we are working with bull data, and yes we did pay attention to evrything when it comes to trimming and quality assessment. but still this is what we have unfortunatelly.

Appreciate your halp

01-31-2020, 04:57 AM	#3
Hedi86 Member Location: Norway Join Date: Oct 2017 Posts: 23	thank you Felix so i just imported the BAM files generated by Bismark into Seqmonk, without any quantification i can click on read length distribution histogram on data sets. but trimmed files should have 20 - 140 bp length, why still i can see seq with for example 400 bp in BAM files? we are working with bull data, and yes we did pay attention to evrything when it comes to trimming and quality assessment. but still this is what we have unfortunatelly. Appreciate your halp