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Old 01-31-2020, 05:00 AM   #4
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Location: Cambridge, UK

Join Date: Sep 2009
Posts: 625

That sounds like you are working with paired-end data. In that case the Read 1 and Read 2 will be ends of the fragment you are sequencing, and they may well be further apart. In this case it doesn't really matter how long each read is, but it is the combination of the mappability of R1 and R2 that counts.
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