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Old 06-04-2018, 06:42 AM   #3
seanw
Junior Member
 
Location: New Brunswick, Canada

Join Date: Nov 2014
Posts: 2
Default Follow up info.

Hi,

here is some clarification on things.

There is no stagger within the primer pairs to increase the diversity and I have not added anything else, but this had not been an issue before (I guess it may be now). I also had a nano run of this library with a greater concentration(30-40%) of PhiX but that still had the issue.

The MoBio/Qaigen subsample library run was a 16S amplicon using the same primer sets that are giving me problem. It was a trial run I used to make sure this was doable with these types of samples.


Here are some screen shots and yes it certianly looks like a diversity issues...
Attached Files
File Type: ppt sequencing.ppt (562.5 KB, 5 views)
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