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Old 09-17-2012, 07:09 PM   #1
rubyryan
Junior Member
 
Location: New Zealand

Join Date: Sep 2012
Posts: 6
Default RNA extraction and DNase treatment

Hi there,
I am planning to carry out a small RNA sequencing project and would like some advice on the RNA extraction/ DNase treatment protocols. One of my plans is to use the MirVana Paris kit followed by Ambion DNase treatment (either Turbo or DNA free kits). Anyone tried these methods? After using the Ambion kits is it necessary to filter purify to remove the DNA removal reagent or is simple pipetting the RNA enough? I really want to avoid any contamination.

My other choice is to use the Norgen RNA extraction kits and do an on-column digestion with Invitrogen. It would avoid any post-RNA extraction procedures but may give a lower yield.
I will perform the small RNA sequencing on the Illumina HiSeq

Any advice/ knowledge would be gratefully appreciated.
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