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  • queries about tophat fusion output

    I have some queries about result.html, http://tophat-fusion.sourceforge.net/data/result.html, explanations of which I do not understand

    9. Number of spanning mate pairs
    10. Number of spanning mate pairs where one end spans a fusion
    11. If you follow the the 9th column, it shows coordinates "number1:number2" where one end is located at a distance of "number1" bases from the left genomic coordinate of a fusion and "number2" is similarly defined

    Am I right to say that 9. represents the number of paired ends, with one end totally on one chromosome of a fusion, and the other totally on the other chromosome of a fusion?

    Would I also be right to say that 10. is the same as 9., except that 9. has no end spanning a fusion, and 10. has one end spanning. Which is to say that 9. and 10. are mutually exclusive.

    Given the above definitions, from the eg in result.html

    4. chr20-chr20 rf

    MCF7
    SULF2
    chr20
    46415145
    ENSG00000171940
    chr20
    52210297
    22
    18
    27

    MCF7
    SULF2
    chr20
    46415146
    ENSG00000171940
    chr20
    52210647
    11
    18
    14

    22 sets the limit on the number of reads spanning a fusion, why is the number 27 greater than 22?

    For 11. is it possible to give an illustration of what exactly you mean? I did the math by adding the genomic coordinate to the number and compared it to the coordinates in the reads section but the numbers do not match up. Also what is the difference between – and + number1 and number2

    Really grateful if you can enlighten me!
    i have tried looking at the documentation but it is cryptic. i hope i am not sounding just at cryptic trying to put across concepts that require illustration.

  • #2
    I also want to get the answer!!!

    Comment

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