Greetings,
I am making a ddRAD library and post-digest and cleanup my concentrations are very low. Since I have to standardize my DNA concentrations pre-PCR I would have to standardize at a very low mass of DNA (10ng per sample) to get the majority of my sequences standardized. If I am for a higher mass of DNA per sample (20ng) I won't be able to meet that level with the majority of my samples.
My question is: is it is better to have more standardized samples at a low DNA mass or less standardized at a higher DNA mass?
Thank you!
I am making a ddRAD library and post-digest and cleanup my concentrations are very low. Since I have to standardize my DNA concentrations pre-PCR I would have to standardize at a very low mass of DNA (10ng per sample) to get the majority of my sequences standardized. If I am for a higher mass of DNA per sample (20ng) I won't be able to meet that level with the majority of my samples.
My question is: is it is better to have more standardized samples at a low DNA mass or less standardized at a higher DNA mass?
Thank you!
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