View Single Post
Old 02-19-2014, 07:47 AM   #1
Sergio.pv
Member
 
Location: Berlin

Join Date: Jul 2013
Posts: 20
Default Is 30000 reads too low for mapping (miRNAs)

Hello community,
I have just received a fastq file of a study i am conducting.
It had an overall poor quality, and after filetering reads according to quality and size (minQV=17, min length=16), the number of reads decreased from 95428 to 29635. And the quality increase markedly.

My question is if it would be still possible to use those ~30000 readss fro mapping.


Best regards and thanks!
Sergio
Sergio.pv is offline   Reply With Quote