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Old 08-30-2018, 03:12 AM   #1
Location: UK

Join Date: Jan 2014
Posts: 75
Default Cost-effective sequencing of >200 20kb amplicons

Dear all, title says it all:

We are looking into the best/most efficient/cost effective way of fragmenting/barcoding/sequencing a ~20kb target/fragment from ~200 independent sources. Final read depth needs to be ~1000x to enable variant calling.

Any ideas?

We were initially looking at NexteraXT, but this will come to over 7000 just for library prep materials for 200 samples.

Does anyone know of a homebrew option that enables multiplexing? Something with barcoded random primers perhaps?

Cheers! Matt
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