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Old 02-08-2013, 12:08 AM   #5
Location: France

Join Date: May 2011
Posts: 25

I tried sonicating at 5kb and 3kb. The sonication profile looks good on the bioanalyzer.

However, the amount of DNA before sonication and after sonication/purification is very different. Indeed, the recovery ratio is not very good.

I'm measuring the DNA concentration with Qubit.

Do you, guys, have the same issue?
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