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Old 07-09-2014, 04:15 AM   #4
tyfach
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Location: London

Join Date: Oct 2011
Posts: 6
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Ah - I see - is this because of the cost or the workflow?
If you are doing custom panels, is it because they are always at least 2 pools (so 2 library kits / 8 samples)? If so, you can cut the volume of the initial PCR to 10ul (still using 10ng DNA per pool) and then combine the 2 pool products and perform the rest of the workflow as if it was a 1 pool panel.
Otherwise, no, I don't think there's an alternative...
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