"Next Generation" does have a nicer ring to it than "Markedly Faster than Last Year (but wait until next year)"
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Which generation?
By my count Sanger is 3rd generation. The 1st nucleic acid sequence I remember was a tRNA sequence obtained by analysis of nuclease products on 2D thin layer chromatography. Then Maxam-Gilbert chemical sequencing emerged as the 1st tractable way to sequence longer stretches of DNA (how many or you know how to pour a meter long, 0.5mm thick polyacrylamide gel with no bubbles?). Then came the Sanger method which was much less finicky than chemical sequencing and ruled for more or less a human generation (albeit with technical improvements like switching to capillary electrophoresis along the way).
Now what you have is the parallel evolution of a variety of different technologies. I think the generational analogy has broken down. Perhaps a speciation analogy is more applicable now. I don't see single molecule sequencing as the next wave per se, rather each technology competing now has a niche application and often two technologies need to be combined to do a particular job well.
Comment
-
Oh, the gels I have poured....
I did not have any bubble problems with acrylamide gels--would simply degas a solution of 5x recrystalized acrylamide before pouring....
Additionally, I've made purified whole protein samples, but never got to perform sequencing on those. Protein sequencing provided us the first glimpse at evolutionary conservation and those isoforms in action. Now we have the short course.
Comment
-
It will be "XGen": Before X PRIZE, After X PRIZE
How to identify sequencing technologies will become straightforward after some team demonstrates they can win the $10M Archon Genomic X PRIZE <http://genomics.xprize.org/>. The stringent requirements of the AGXP will distinguish all platforms capable of achieving such goals and henceforth sequencing platforms would be identified as “X PRIZE Capable” versus those that are not.
Larry Kedes
Senior Advisor. X PRIZE Foundation
Scientific Director, Archon Genomic X PRIZE
Comment
-
Originally posted by Michael.James.Clark View Post
ECO, I actually think you might be okay leaving "next generation" in the tag line. I mean, we are always going to be discussing the "next generation" (plus some of the last generation, probably).
Comment
Latest Articles
Collapse
-
by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
Channel: Articles
04-04-2024, 04:25 PM -
-
by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
Channel: Articles
03-22-2024, 06:39 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 04-11-2024, 12:08 PM
|
0 responses
25 views
0 likes
|
Last Post
by seqadmin
04-11-2024, 12:08 PM
|
||
Started by seqadmin, 04-10-2024, 10:19 PM
|
0 responses
29 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 10:19 PM
|
||
Started by seqadmin, 04-10-2024, 09:21 AM
|
0 responses
24 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 09:21 AM
|
||
Started by seqadmin, 04-04-2024, 09:00 AM
|
0 responses
52 views
0 likes
|
Last Post
by seqadmin
04-04-2024, 09:00 AM
|
Comment