Hi All,
I need to align ABI Solid reads to just 1 gene to see how many reads align to just that gene. (No mapping has been done so far). I need to know what is the best way to do this. It seems like an over kill for me to create a reference file, index etc for 1 gene and then align the read files using any aligner (I am leaning towards NovoalignCS but other suggestions with reasons are welcome). Is there a simpler method to do this kind of single gene alignment for reads?
Thanks,
Vishal
I need to align ABI Solid reads to just 1 gene to see how many reads align to just that gene. (No mapping has been done so far). I need to know what is the best way to do this. It seems like an over kill for me to create a reference file, index etc for 1 gene and then align the read files using any aligner (I am leaning towards NovoalignCS but other suggestions with reasons are welcome). Is there a simpler method to do this kind of single gene alignment for reads?
Thanks,
Vishal
Comment