View Single Post
Old 07-28-2015, 06:25 PM   #3
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
Default

An easy option is to use indexes with 6 bases from a well-established kit and add 6 random bases to follow index read. By doing a 12 cycle index read one can identify PCR dups based on those 6 random bases. For more diverse UMI one can add 8 random bases and increase sequencing index to 14 cycles. Obviously, this is applicable to Y adapters and library must be amplified with short P5 and P7 primers.

Last edited by nucacidhunter; 07-28-2015 at 08:09 PM.
nucacidhunter is offline   Reply With Quote