SEQanswers - View Single Post - Bias in unique molecular identifier usage

donquijotes · 07-30-2015, 06:46 AM

Hi nucacidhunter,

Thank you for your input. What I've seen many people do out there is read the UMI as part of their DNA library insert and not as part of the index. I still don't know how Agilent does this with their HaloPlex HS.

I saw few software out there that can find the UMI and add it in the header (TagDust2 and MiGec for example) but I don't have a clue how to proceed from that point and get rid of PCR duplicates...

Any idea? I'll start a thread since I am rather clueless with the whole procedure.

07-30-2015, 06:46 AM	#4
donquijotes Junior Member Location: Michigan Join Date: Jul 2015 Posts: 7	Hi nucacidhunter, Thank you for your input. What I've seen many people do out there is read the UMI as part of their DNA library insert and not as part of the index. I still don't know how Agilent does this with their HaloPlex HS. I saw few software out there that can find the UMI and add it in the header (TagDust2 and MiGec for example) but I don't have a clue how to proceed from that point and get rid of PCR duplicates... Any idea? I'll start a thread since I am rather clueless with the whole procedure.