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Old 09-26-2016, 04:55 PM   #10
SAV
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Location: San Diego

Join Date: Nov 2012
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Please see this publication showing nextera XT being prepared in 2, 4 and 8 uL and results for single cell RNAseq being compared and showing no loss of complexity.
http://jla.sagepub.com/content/early...216630741.full

And this one having samples prepared using same method but not mentioned in detail in methods.
http://www.cell.com/cell/pdfExtended...674(16)30740-1
libraries were normalized and prepared in 4 uL total volume.
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