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Old 08-04-2017, 08:49 AM   #15
Arthur45
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Location: London

Join Date: May 2017
Posts: 7
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Hi Simone,

thank you for your answer. I experimented with scaling-down the Nextera protocol, with mixed results.

My main worry is that the transposome activity is not behaving as expected. See the TapeStation traces, before and after tagmentation. The 1kb peak has disappeared during tagmentation, which is good news (we are aiming for a 300bp library). But I fail to be convinced that there is any DNA left after tagmentation, looking at the TapeStation electropherogram.

What do you think about it?

Cheers,

arthur
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