Hello All,
I am currently working on a protocol where I will be using NEBNext® Ultra™ II FS DNA Library Prep Kit (Cat # E7805) ligation mix and enhancer as a 2x master mix for adapter ligation.
Now I am including 20x the amount of adapter into the reaction but I am only getting about 30% ligation of my sample. I am looking for a target that is only present in 1/1000 of the time.
Does anyone have any recommendation for using the Ligation mix that NEB provides with this kit?
I am currently working on a protocol where I will be using NEBNext® Ultra™ II FS DNA Library Prep Kit (Cat # E7805) ligation mix and enhancer as a 2x master mix for adapter ligation.
Now I am including 20x the amount of adapter into the reaction but I am only getting about 30% ligation of my sample. I am looking for a target that is only present in 1/1000 of the time.
Does anyone have any recommendation for using the Ligation mix that NEB provides with this kit?
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