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Old 07-04-2013, 08:16 AM   #4
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Location: Germany

Join Date: Jul 2013
Posts: 9

Thanks, but the example reads I've posted are not mine. they are from the wikipedia page illustrating the differences between the fastq-formats.
But then how can I check the assemblies with regard to paired-end Infomation? With Mira, for example, I know that you still get assemblies (without error-messages) if you use the wrong format. The assemblies will just in reality be based on single-read assemblies. So how can one really make sure if everything is ok, if the paired-end info is left out from the AMOS-file, the sequence-list and everything? (This just intrigues me. For some contigs i would simply like to check how well supported they are by paired reads. Also a lot of reads are not assembled or dropped from the assembly. I would like to see what has happened to their corresponding partners)

Last edited by someperson; 07-04-2013 at 08:29 AM.
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