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Old 05-07-2016, 08:29 AM   #4
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Location: East Coast USA

Join Date: Feb 2008
Posts: 6,978

Qubit for sample concentration along with bioanalyzer for size estimation is essential to get balanced pools. You will always find some variation in the actual number of reads you get for each sample (due to pipetting/conc estimation errors).

As you discovered yourself smaller inserts (adapter dimers qualify) cluster very efficiently compared to longer real inserts. So the only way to avoid that is to get rid of them by purification.
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