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Old 12-17-2018, 08:42 PM   #1
aharring
Junior Member
 
Location: Nevada

Join Date: Dec 2018
Posts: 2
Default Few questions about minion

I want to obtain long read data for 6 bacterial isolates. I have purchased the minion starter kit with the ligation kit and native barcode kit. I am confused on why the protocol requires the Blunt/TA ligase master mix and the NEBNext Quick Ligation Module, it seems like its redundant. Could I just use the NEBNext Quick Ligation Module which does blunt and TA ligation for both barcoding and adding the adapter?

From what I understand is that I do the dA tailing on my DNA, ligate unique barcodes to each sample, pool the different barcodes together at a certain concentration and ligate the adapters.
Last question, how necessary is the qubit? I only have a nanodrop and was curious if anyone has prepped a library using a nanodrop only.
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