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Old 04-07-2019, 02:48 PM   #3
gringer
David Eccles (gringer)
 
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 836
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Qubit or Quantus (or other similar fluorescence-based methods) are pretty much essential for nanopore sequencing. The nanodrop has issues with RNA contamination, and gives concentrations that can be a couple of orders of magnitude higher than the true DNA concentration.

You can make do with just a nanodrop, but you need to take a lot more care with making sure that the DNA is as pure as possible. I wouldn't recommend nanodrop-only when starting out with nanopore sequencing.
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