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Old 11-10-2010, 06:29 AM   #26
TonyBrooks
Senior Member
 
Location: London

Join Date: Jun 2009
Posts: 298
Default Total RNA sequencing

Hi all
Has any one tried a random primed approach on total RNA with a DSN normalisation?
Hopefully we'll be able to remove most rRNA from the samples, but keep the non polyA RNAs that could still be interesting. Alternatively, has anyone validated the Roche rapid cDNA kit from mRNA with a DSN normalisation after end repair?
Thanks in advance
Tony
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