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Old 12-03-2010, 04:19 AM   #28
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Location: Purdue University, West Lafayette, Indiana

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Just to be clear: we do not modify the SMART procedure in the way I describe above. I just mentioned it as a possible solution to a problem we do not seem to have but does plague others.

About the addition of an oligo C tail to the reverse transcribed strand: that is the basis of second strand synthesis in the SMART kit. SMART uses an oligo G tailed second strand synthesis primer.

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