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Old 12-13-2010, 04:25 AM   #2
pmiguel
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Location: Purdue University, West Lafayette, Indiana

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Plus and minus amplification adaptors? In both cases it looks like the difference in size is about 70 bp. This would require your using a very small number of amplification steps after ligation, or starting with a surfeit of DNA in your ligation.

What instrument are these libraries constructed for?
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