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Old 01-05-2011, 03:55 AM   #34
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,313
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Hi Martin2,
Yeah, I suppose were I to spend a few weeks probing the muddy depths into which Roche has sunk their gsRunProcessor pipeline I might be able to divine their intent. But thanks for your outline, I do appreciate it. This is exactly the sort of discussion I think useful. (I dare say it might happen more frequently if Roche did not feel the need to hide their manuals away from the non-devout.)
I suspect most of the issues we were having when I posted (more than 1 year ago) what you quote had to do with the 454s particular susceptibility to homopolymers. Even now the only way I know to discover if a library is "poisoned" by long polyA tails is to fire up gsRunBrowser and iterate through the early images of a run.

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Phillip
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