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Old 11-08-2012, 02:21 PM   #19
Nanoporous
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Location: San Diego

Join Date: Dec 2009
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Quote:
Originally Posted by ECO View Post
Overall my conversation was pretty disappointing as far as new info (although the gentleman I talked to resisted a pretty good grilling from me and a 454/Roche product manager).

Points from my conversation that I can remember...
  • GridION == "4-5x more sensors" than the MinION (no comparative runtime specs).
  • GridION can sip from a 96 well plate full of samples (this was demo'd as a flat bottom black microtiter plate with no septa...hmmm).
  • Acquisition speed == "bases per second".
  • The above picture suggesting sample prep is just a "10bp overhang" is not correct and not confirmed
  • The prototypes of the MinION ended up much larger than shown at AGBT due to heat issues (had to "add fans").
  • They will not confirm which gating protein (exo or phi29) or which pore (mspA or a-HL), but that the gate protein is not covalently coupled to the pore. (which strongly suggests to me that it is phi29...the exo-release method would undoubtedly need exquisite positioning for efficient detection)
  • According to the CEO, the DNA input concentration is "nanomolar". This makes sense to be given the binding kinetics of phi29 to it's binding site...but translates into high concentrations for a single molecule platform. When questioned about the possibility of doing very dilute samples where all the molecules matter, he suggested some method of on-chip enrichment/concentration (?????).
  • They are cagey about data release because they want to (my paraphasing) "release data when it's ready"
Anyway, that's all I got. The ratio of ([booth girls] + [Apple-esque brushed aluminum] + [blinky lights]) to (data) was undefined...so that seems to speak for itself.
Thanks for the very nice summary.

For the 'gate' protein, I think they are using some sort of a motor protein to slow down the motion of the DNA in a stepwise manner. As per Clive Brown's talk at AGBT, it is certainly not attached to the pore, and that it is definitely not phi29 (which is what the Akeson and Gundlach groups are using). A polymerase like that would require that you add NTPs. So probably a helicase or something.

Last edited by Nanoporous; 11-08-2012 at 03:27 PM.
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