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Old 04-05-2013, 11:10 AM   #1
Location: Michigan

Join Date: Jul 2011
Posts: 10
Default kmer content warning in FastQC

I would be so grateful if someone wouldn't mind taking a look at the kmer content graph from FastQC. This is RNA-seq data after trimming and filtering based on quality scores. My questions are: Does the y-axis represent frequency? If it does why are these kmers so frequently found? What could they be? Transposons? DNA? I should mention, that there are no overrepresented sequences detected by FastQC.

Thank you in advance for your time!!!
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