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MU Core · 12-29-2015, 07:25 AM

I assume you are using Big Dye v3 chemistry. I would expect the reaction to work as long as there were no other issues (i.e. primer is annealing, GC-rich template problems, etc.).

12-29-2015, 07:25 AM	#2
MU Core Member Location: Columbia, Missouri Join Date: Apr 2008 Posts: 57	I assume you are using Big Dye v3 chemistry. I would expect the reaction to work as long as there were no other issues (i.e. primer is annealing, GC-rich template problems, etc.).