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Old 03-07-2011, 10:16 AM   #1
Bruce E
Location: Rochester,MN

Join Date: Jan 2008
Posts: 15
Default Fastqc sequence duplication levels

Our informatics group recently started to use fastqc. I am seeing high (70%) sequence duplication for TruSeq mRNA-Seq libraries. These are from a single lane of a 51 bp read on Illumina's HiSeq 2000. I also see ~30-40% duplicates from a exon cature run. Is this normal? I am concerned that it is way too high.
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File Type: pdf mRNA-seq Sequence Duplication Levels.pdf (106.3 KB, 200 views)
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