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Old 01-03-2012, 09:28 AM   #7
Location: US

Join Date: Oct 2011
Posts: 47

Here are the answers to your questions. Thanks so much again. I will wait for the replies.

1. Do you have a particular organism that you are working with?
- Human

2. Do you have the fasta file of the genes that you want the lengths of?
- No

3. Do you want the length of the cDNA or the genes with introns?
- The data is generated using Illumina (Exome sequencing)
- So I understand I need the length of all exons in a gene (how would I get this info for a set of 1000 genes?). Am I right?

4. Do you care about splice variants or will the longest splice variant?
- well, I don't know the answer. Should I consider the entire exon length as the length of the longest transcript?
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