Hi all,
Pretty simple question here. I recently did ATAC seq on 10 samples, they have adapters and are all uniquely barcoded. I’m about to pool to prepare for NGS submission. I was told that the ideal concentration to submit was 10-20nM in approx 20uL & that I should make everything equimolar before pooling. The problem is that most of my samples range from 4-8nM, while only one is above 10nM. Will I need to make the final pooled mixture 4nM since this is my least concentrated sample? I am essentially limited by the sample of lowest concentration, correct? Just making sure I’m not missing something here. Thanks
Pretty simple question here. I recently did ATAC seq on 10 samples, they have adapters and are all uniquely barcoded. I’m about to pool to prepare for NGS submission. I was told that the ideal concentration to submit was 10-20nM in approx 20uL & that I should make everything equimolar before pooling. The problem is that most of my samples range from 4-8nM, while only one is above 10nM. Will I need to make the final pooled mixture 4nM since this is my least concentrated sample? I am essentially limited by the sample of lowest concentration, correct? Just making sure I’m not missing something here. Thanks
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