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Old 02-29-2016, 08:08 PM   #20
Maflores11
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Location: Raleigh, NC

Join Date: Feb 2016
Posts: 3
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Hi Kathi_seq,

This is Miguel Flores (user: ashes11), I was unable to login in with my previous account.

In order to minimize COCl2 precipitation (likely due to DTT in NEB4 Buffer), I tried less concentration of COCl2 until I found a balance. I recommend to run an experiment with titrations of COCl2. I should probably contact my former adviser to check my notes to get specifics. LinDA worked in my hands, but requires some tweaking.

Cheers!
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