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Old 08-16-2016, 01:57 PM   #4
dpryan
Devon Ryan
 
Location: Freiburg, Germany

Join Date: Jul 2011
Posts: 3,480
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Hi Ted, do you have any data regarding de novo transcriptome assembly (e.g., with trinity) of RNAseq data with sequins spike-ins? I imagine you could use the K562 data for that (at least from an initial read of the Hardwick et al. paper). We sometimes need to do RNAseq and transcriptome assembly of non-model organisms where people are explicitly interested in things like splicing. It'd be really nice to be able to use something like sequins to assess how much we might be missing (and assembly artefacts of course).
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