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Old 10-16-2018, 01:02 AM   #4
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,248

I do not know how the library has been prepped but I notice following:

1- It is stranded (directional) library
2- Probably RNA has been poly adenylated after extraction
3- It contains some short fragments (below 100 base)
4- RT reaction probably has been primed with polyT primer

It is library prep issue and not the sequencing. If you would like help you need to give more info on extraction and library prep.
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