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Old 05-05-2019, 07:20 AM   #2
Bukowski
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Location: UK

Join Date: Jan 2010
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How many groups in the experiment? How many samples per group? Are they paired samples (i.e. pre and post treatment?) What's the actual experimental setup? What question are you trying to answer? Where your samples RNA extracted on the same day? Libraries prepped on the same day? With the same reagents? What possible batch effects are there in your setup?

Very hard to advise on anything other than to argue that biological replicates are important and technical replication largely unecessary.
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