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Old 03-16-2020, 07:51 AM   #4
olafblue1955
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Location: Madison

Join Date: Feb 2019
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Hi-

OK, that's fine. We know of researchers who do the rRNA depletion as a precursor to capture as it is know that there are rRNA sequences similar to regions in mammalian RNA:

Mauro, et al. PNAS 94:422-427 (January 1997):

"rRNA-like sequences occur in diverse primary transcripts:
Implications for the control of gene expression"

Do your rRNA sequences actually map to the 5s/5.8s/18s/28s sequences for your organism in the reference genome you're using?

Olaf
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