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Old 09-27-2021, 09:53 PM   #2
Chaos Indeterminate
Junior Member
 
Location: India

Join Date: Sep 2021
Posts: 2
Post Differential expression of mRNAs and microRNAs on the Illumina HiSeq platform.

Hi,

I thought I will add my query as well here.

I am new to this group and have zero experience with sequencing. We want to find the differential expression of mRNAs and microRNAs on the illumina HiSeq platform.

Can anyone suggest the best protocol for library preparation that will enable to get realistic data for both mRNA and microRNA sequences and abundance from a single protocol that will minimize the expenditure.

Also this is mouse or human, so what I learnt so far unidirectional sequencing maybe sufficient and no need for paired ends right? Also when I say unidirectional do I always mean the 5' end or the 3' end because we want microRNAs also that ay be important. So which unidirectional protocol should we follow, 5' or 3'??

I hope the questions make sense. Please do reply and help.

Thanks!!
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