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Old 04-11-2013, 10:49 AM   #14
thomasblomquist
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Location: Ohio

Join Date: Jul 2012
Posts: 68
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Barcode contamination from the synthesis and preparation steps is a HUGE issue. A lot of care for stringency in all steps must be taken. Don't use plates to prep sequencing libraries in parallel!!!

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245947/

Also,

http://www.idtdna.com/pages/support/...ade-processing

Be careful with barcoding and multiplexing. As with any experiment, include controls, etc. to monitor level of cross-contamination -talk between barcodes, etc.

-Tom
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