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Old 01-27-2014, 12:01 PM   #2
Location: Dallas

Join Date: Aug 2012
Posts: 21

Please ignore my initial post. The real reason why my read is not mapped is not the "N", it's because in the FLAG field it's marked as "0x10 SEQ being reverse complemented". When I change this single bit to 0, the read got counted. Does any body have any idea?
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