Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Any aftermarket 454 reagents being (or going to be) produced?

    So when the Roche 454 reagents dry up, will no other company step up with compatible kits? I notice Epicentre has dropped all their Nextera stuff.

    Will Roche make the reagents public in case we want to try and make our own kits? We can pretty much guess at a lot of the protocols, but it would be great to just have a cookbook method available.

    I asked about "repurposing" the 454-Junior we have, but no ideas so far.

  • #2
    I haven't heard anything yet, but we are hoping the same thing.

    Epicentre is slowly shifting everything to Illumina, at least Illumina picked up the Nextera.

    Comment


    • #3
      Reagents are one thing, but I can't imagine anyone would want to lay out for manufacturing picotiter plates.

      Comment


      • #4
        We can get the picotitre plates pretty clean with washing and sonicating. I know labs that have reused them. Also, the channel glass they are made from has been around for a couple decades now (before 454 technology) and will still be made.

        Comment


        • #5
          Originally posted by hoytpr View Post
          We can get the picotitre plates pretty clean with washing and sonicating. I know labs that have reused them.
          And that is exactly what ruined a number of projects. I spent insane amount of time cleaning up contaminated projects. Don't do it. Although some labs could be more thorough in sonication and water exchange, clearly the beads not only do remain in the PTP plate but even the enzymes keep working in a following sequencing. Feel free to place an order on my website www.bioinformatics.cz for cleanup of your current data. ;-)

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Strategies for Sequencing Challenging Samples
            by seqadmin


            Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
            03-22-2024, 06:39 AM
          • seqadmin
            Techniques and Challenges in Conservation Genomics
            by seqadmin



            The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

            Avian Conservation
            Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
            03-08-2024, 10:41 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, Yesterday, 06:37 PM
          0 responses
          8 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, Yesterday, 06:07 PM
          0 responses
          8 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 03-22-2024, 10:03 AM
          0 responses
          49 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 03-21-2024, 07:32 AM
          0 responses
          66 views
          0 likes
          Last Post seqadmin  
          Working...
          X