View Single Post
Old 04-02-2012, 04:59 PM   #2
NextGenSeb
Member
 
Location: Melbourne

Join Date: Jan 2012
Posts: 15
Default

Hi Sarah,
I have had a couple of runs on the v1 ampliseq cancer panel, with mixed results re: amplicon coverage. Some appear to drop out systematically, however I understand that is a well known issue with the panel. The new v2 panel is supposed to fix that, but I have just started testing that so can't comment there. As for the data quality, at this stage it seems to be lower than what you get of a MiSeq or related system, especially single base deletions seem overly frequent. But it is a lot faster and at this stage at least the most widely available option.

As for the samples, I am using 314 chips, hence only one sample per run. There is the option to run more on a bigger chip though.

Cheers
Seb
NextGenSeb is offline   Reply With Quote