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Old 01-11-2013, 12:49 PM   #16
ashes11
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Location: North Carolina

Join Date: Dec 2012
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It seems that the DTT in NEB Buffer 4 precipitates COCl2 in the reaction solution. T-tailing of an internal control (50 ng PCR fragment, 360 bp) does not work in my hands when using NEB Buffer 4, 5mM COCl2 and NEB TdT enzyme. Here more proof from Schreiber Lab:
http://www.broadinstitute.org/chembi...ement/FAQ.html
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