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Old 04-23-2020, 10:30 AM   #9
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Location: Boston, MA, USA

Join Date: Apr 2020
Posts: 5

Originally Posted by cement_head View Post
I'd have to agree with GenoMax; super-important to have a consultation with the sequencing center about the library composition and ask them what they recommend. You probably should have had 10% PhiX spike-in added. HiSeq are terrible at dynamic calibration - MiSeqs are better (to a point).
I see. Next time I will consider PhiX spike-in. Thanks, cement_head!
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