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Old 07-23-2014, 03:45 PM   #16
Location: Prague, Czech Republic

Join Date: Nov 2010
Posts: 40

Hi pmart1,
sorry I somehow did not receive an email update.

The assembly you show is bad, 95% of reads having no edge means it just did not assemble, almost at all. Was this only the 454 data or 454 with Illumina together? Try to assemble just the 454 data alone.

Are you sure you removed adapters? You should really go to the lab protocols to check what you have in the files. Or ask somebody knowing how to check the raw data. It is hard to tell remotely. Provided it is your special strain and top-secret ... I could help you debug the data via private email, depends how much info you can disclose.

martin2 is offline   Reply With Quote